Honey and Nigella sativa L has been known for millennia of years for its therapeutic healing properties. This
present histopathological study aims to evaluate the healing activity of Algerian bee honey derived from the
nectar of Thymus vulgaris mixed with N. sativa seed powder on a sheep model. Induction of wounds with
longitudinal incisions of full thickness, was carried out in the dorsal part of 09 healthy sheep belonging to the
animal facility of the veterinary department of the University of Batna 1. After inductions of the wounds, the
animals were distributed to the randomly in 03 batches of animals of 03 sheep for each with appropriate topical
care for each batch: the batch of animals treated with Thymus vulgaris honey mixed with N. sativa seed powder
(HNS), the Madicassol batch and the Vaseline. The topical application of the various treatments was carried out
once a day for 02 successive weeks. Histopathological evaluation was performed by measuring scar tissue
thickness. In the batch of animals treated with HNS mixture, the thickness of the scar tissues revealed a
significant decrease compared to the Madicassol and Vaseline batches. No keloid or detectable in the wounds of
the mixture HNS lot. The results of this experimental study suggested that the topical use of local honey from the
nectar of T.vulgaris mixed with N. sativa seed powder significantly reduced the thickness of wound healing in
sheep.

Background and Aim: This investigation is the continuation of a published preliminary study examining the therapeutic
efficacy of platelet-rich plasma (PRP) as a topical treatment for skin wounds in sheep. The study aimed to compare the
healing effects of autologous PRP with that of natural honey.
Materials and Methods: This study involved nine clinically healthy male sheep. After sterile skin preparation, fullthickness
longitudinal incision wounds were created on the backs of each animal. The animals were randomly divided into
three groups of three sheep each. In Group I, the wounds were treated with PRP; in Group II, the wounds were treated with
honey; and in Group III, the wounds were treated with saline solution. The different treatments were administered topically
every 3 days. Healing was assessed by a semi-quantitative histopathological study from biopsies taken on the 3rd, 7th, 14th,
21st, and 28th days of healing. The data obtained were compared using the non-parametric Mann–Whitney U-test, and p<0.05
and 0.01 were used to determine the level of significance of the recorded differences.
Results: Semi-quantitative histopathological evaluation showed significant differences in the progression of wound healing
between the three study groups. Recorded data showed that PRP may reduce inflammation during the first 3 days after the
incision. Moreover, the synthesis and organization of collagen fibers were significantly improved in the group treated with
PRP compared with those in the group treated with honey.
Conclusion: PRP offers a promising therapeutic option for healing skin wounds in sheep compared with honey.
Keywords: honey, platelet-rich plasma, sheep, skin, wound healing.

Aim: The therapeutic evaluation of the biological effect of platelet-rich plasma (PRP) used as a surgical adjunct to maintain
the inflammatory process and to potentiate tissue healing, make the subject of recent research in regenerative medicine. This
study was designed to evaluate the healing activity of PRP by its topical application on the skin experimentally injured in
a sheep model.
Materials and Methods: The study was conducted on 9 adult and clinically healthy males sheep. PRP was obtained by a
protocol of double centrifugation of whole blood from each animal. After sterile skin preparation, full-thickness excisional
wounds (20 mm x 20 mm) were created on the back of each animal. The animals were randomly divided into three equal
groups of three sheep for each. In Group I, the wounds were treated with PRP, in Group II; wounds were treated with
Asiaticoside; in Group III, wounds were treated with saline solution. The different treatments were administered topically
every 3 days. Morphometric measurements of the contraction surface of the wounds and histopathological biopsies were
carried out at the 3rd, 7th, 14th, 21st, and 28th days of healing.
Results: The results of the morphometric data obtained revealed that it was significant differences recorded at the 7th and
14th day of healing in favor for animals of Group I. Semi-quantitative histopathological evaluation showed that PRP reduces
inflammation during 3 first days post-surgical and promotes epithelialization in 3 weeks of healing.
Conclusion: We concluded that topical administration of PRP obtained by double centrifugation protocol could potentially
improve the skin healing process in sheep.

Abstract: The therapeutic evaluation of the biological effect of platelet-rich plasma (PRP) is the subject of
several studies in regenerative medicine. The aim of this study was to compare four different methods of
preparation of autologous platelet concentrates in sheep. Various specimens were collected from six adult
healthy male sheep of the local breed. Blood smears were also carried out on all the samples, in order to see the
morphology of the platelets. The data obtained in the manual platelet count were submitted to statistical
analysis (repeated measures ANOVA, Tukey HSD test, P<0.05). The result was that the total mean number of
platelets in whole blood was 521.56 ± 97.01x10 /µl. The PRP samples in Groups I and III showed significantly 3
higher mean platelet counts than Group II and IV (1438.25 ± 221.72×10 /µl and 1430.75 ± 293.63×10 /µl, 3 3
respectively). In conclusion, within the limit of this study, it can be concluded that the best technique of platelet
harvesting in sheep is obtained with the double centrifugation protocol used for animals of group I.