@article {105268,
	title = {GC-Ms, UHPLC\&ndash;MS/MSn Phytoconstituents Characterization and Physio-pharmacological Investigation of Silene colorata subsp. amphorina (Pomel) Batt. From Northeastern Algeria},
	journal = {Chemistry \& Biodiversity},
	volume = {23},
	number = {8},
	year = {2026},
	pages = {e71578},
	abstract = {Natural remedies combined with bioinformatics accelerate the formulation of safer, targeted drugs. This study provides a comprehensive description of the phytochemical and pharmacological aspects of Silene colorata. Binding profiles of eight identified compounds were simulated against α-amylase. GC-MS analysis revealed 57 compounds, mainly fatty acids and esters (73.65\%), while UHPLC{\textendash}MS/MS identified 2',4'-dihydroxychalcone (20.72\%), (epi)gallocatechin-C-hexoside (12.53\%), apigetrin (10.10\%), and hexahydroxyheptanoic acid (8.57\%) as major components. The hydro-alcoholic extract showed a strong antiradical effect (IC50 = 7.57 {\textpm} 0.26 {\textmu}g/mL by ABTS assay; A0.50 = 48.93 {\textpm} 3.88 {\textmu}g/mL by phenanthroline assay). Its sun protection factor (SPF) was also the highest (40.561 {\textpm} 0.87). The chloroform fraction showed notable activity against α-amylase (IC50 = 22.86 {\textpm} 3.19 {\textmu}g/mL) and butyrylcholinesterase (IC50 = 111.08 {\textpm} 4.72 {\textmu}g/mL). The complex 3-tetradecyn-1-ol and α-amylase appeared more stable, but in an antagonistic manner (-4.8 kcal/mol). While this work reveals crucial findings, further in vivo studies are needed to confirm and expand on these results.},
	url = {https://onlinelibrary.wiley.com/doi/10.1002/cbdv.71578},
	author = {Bensaad, Mohamed Sabri and Allaoua, Zina and Kahoul, Mohamed Amine and Diana C. G. A. Pinto and Chawki Bensouici and Salah Akkal}
}
