@article {73704,
	title = {Histological assessment of topical mixture of Thymus vulgarishoney and Nigella sativa L seed powder on skin wound healingin sheep},
	journal = {international of biosciences },
	volume = {21},
	number = {1},
	year = {2022},
	abstract = {Honey and Nigella sativa L has been known for millennia of years for its therapeutic healing properties. Thispresent histopathological study aims to evaluate the healing activity of Algerian bee honey derived from thenectar of Thymus vulgaris mixed with N. sativa seed powder on a sheep model. Induction of wounds withlongitudinal incisions of full thickness, was carried out in the dorsal part of 09 healthy sheep belonging to theanimal facility of the veterinary department of the University of Batna 1. After inductions of the wounds, theanimals were distributed to the randomly in 03 batches of animals of 03 sheep for each with appropriate topicalcare for each batch: the batch of animals treated with Thymus vulgaris honey mixed with N. sativa seed powder(HNS), the Madicassol batch and the Vaseline. The topical application of the various treatments was carried outonce a day for 02 successive weeks. Histopathological evaluation was performed by measuring scar tissuethickness. In the batch of animals treated with HNS mixture, the thickness of the scar tissues revealed asignificant decrease compared to the Madicassol and Vaseline batches. No keloid or detectable in the wounds ofthe mixture HNS lot. The results of this experimental study suggested that the topical use of local honey from thenectar of T.vulgaris mixed with N. sativa seed powder significantly reduced the thickness of wound healing insheep.},
	author = {deffa ouafa and DAIKH Badis}
}
@article {73703,
	title = {Comparative study of the therapeutic efficacy of autologousplatelet-rich plasma and honey in healing skin wounds in sheep},
	journal = {veterinary wourd},
	volume = {14},
	number = {8},
	year = {2021},
	abstract = {Background and Aim: This investigation is the continuation of a published preliminary study examining the therapeuticefficacy of platelet-rich plasma (PRP) as a topical treatment for skin wounds in sheep. The study aimed to compare thehealing effects of autologous PRP with that of natural honey.Materials and Methods: This study involved nine clinically healthy male sheep. After sterile skin preparation, fullthicknesslongitudinal incision wounds were created on the backs of each animal. The animals were randomly divided intothree groups of three sheep each. In Group I, the wounds were treated with PRP; in Group II, the wounds were treated withhoney; and in Group III, the wounds were treated with saline solution. The different treatments were administered topicallyevery 3 days. Healing was assessed by a semi-quantitative histopathological study from biopsies taken on the 3rd, 7th, 14th,21st, and 28th days of healing. The data obtained were compared using the non-parametric Mann{\textendash}Whitney U-test, and p\&lt;0.05and 0.01 were used to determine the level of significance of the recorded differences.Results: Semi-quantitative histopathological evaluation showed significant differences in the progression of wound healingbetween the three study groups. Recorded data showed that PRP may reduce inflammation during the first 3 days after theincision. Moreover, the synthesis and organization of collagen fibers were significantly improved in the group treated withPRP compared with those in the group treated with honey.Conclusion: PRP offers a promising therapeutic option for healing skin wounds in sheep compared with honey.Keywords: honey, platelet-rich plasma, sheep, skin, wound healing.},
	author = {DAIKH Badis}
}
@article {18433,
	title = {The effectiveness of platelet-rich plasma on the skin wound healingprocess: A comparative experimental study in sheep},
	journal = {Veterinary World,},
	volume = {Vol.11},
	number = {2231-0916},
	year = {2018},
	pages = {800-808},
	abstract = {Aim: The therapeutic evaluation of the biological effect of platelet-rich plasma (PRP) used as a surgical adjunct to maintainthe inflammatory process and to potentiate tissue healing, make the subject of recent research in regenerative medicine. Thisstudy was designed to evaluate the healing activity of PRP by its topical application on the skin experimentally injured ina sheep model.Materials and Methods: The study was conducted on 9 adult and clinically healthy males sheep. PRP was obtained by aprotocol of double centrifugation of whole blood from each animal. After sterile skin preparation, full-thickness excisionalwounds (20 mm x 20 mm) were created on the back of each animal. The animals were randomly divided into three equalgroups of three sheep for each. In Group I, the wounds were treated with PRP, in Group II; wounds were treated withAsiaticoside; in Group III, wounds were treated with saline solution. The different treatments were administered topicallyevery 3 days. Morphometric measurements of the contraction surface of the wounds and histopathological biopsies werecarried out at the 3rd, 7th, 14th, 21st, and 28th days of healing.Results: The results of the morphometric data obtained revealed that it was significant differences recorded at the 7th and14th day of healing in favor for animals of Group I. Semi-quantitative histopathological evaluation showed that PRP reducesinflammation during 3 first days post-surgical and promotes epithelialization in 3 weeks of healing.Conclusion: We concluded that topical administration of PRP obtained by double centrifugation protocol could potentiallyimprove the skin healing process in sheep.},
	url = {www.veterinaryworld.org},
	author = {DAIKH Badis and BENOUNE Omar}
}
@article {18435,
	title = {Comparative Study of Four Methods of Preparationof Autologous Plasma-Rich Platelet in Sheep},
	journal = {Global Veterinaria},
	volume = {6},
	number = {17},
	year = {2016},
	pages = {559-563},
	abstract = {Abstract: The therapeutic evaluation of the biological effect of platelet-rich plasma (PRP) is the subject ofseveral studies in regenerative medicine. The aim of this study was to compare four different methods ofpreparation of autologous platelet concentrates in sheep. Various specimens were collected from six adulthealthy male sheep of the local breed. Blood smears were also carried out on all the samples, in order to see themorphology of the platelets. The data obtained in the manual platelet count were submitted to statisticalanalysis (repeated measures ANOVA, Tukey HSD test, P\&lt;0.05). The result was that the total mean number ofplatelets in whole blood was 521.56 {\textpm} 97.01x10 /{\textmu}l. The PRP samples in Groups I and III showed significantly 3higher mean platelet counts than Group II and IV (1438.25 {\textpm} 221.72{\texttimes}10 /{\textmu}l and 1430.75 {\textpm} 293.63{\texttimes}10 /{\textmu}l, 3 3respectively). In conclusion, within the limit of this study, it can be concluded that the best technique of plateletharvesting in sheep is obtained with the double centrifugation protocol used for animals of group I.\&nbsp;},
	url = {https://www.idosi.org/gv/gv.htm},
	author = {DAIKH Badis and BENOUNE Omar}
}
