We analyzed the influence of land use and water physical-chemical characteristics on the trait composition of benthic macroinvertebrates in arid-land streams of North-East Algeria. Macroinvertebrates were sampled in the spring season of 2015, 2017 and 2018 at 36 sampling sites distributed along 5 streams of the Belezma biosphere reserve. Samples were taken from the various substratum types using a Surber net. Most of the variability of the trait-environment relationship was explained by increasing temperature and conductivity along the downstream gradient. Whilst agriculture at higher elevations did not have a great influence on the functional trait composition of macroinvertebrate communities, agriculture and urbanization at lower elevations generated significant deviations from predictable functional structures. Owing to the natural downstream decrease in community diversity in streams of the study region, entire taxa and/or functional groups were more likely to be wiped out in response to anthropogenic perturbations at lower elevations. Despite human activities, climate-related variables in arid lands play a major role on hydrological regimes that effect instream habitats, water chemistry, and macroinvertebrate communities. Given the environmental constraints in arid-land streams of North Africa, even slight increases in anthropogenic pressure can have negative effects on the taxonomic and functional composition of macroinvertebrate communities.