Abstract:

Abstract: The therapeutic evaluation of the biological effect of platelet-rich plasma (PRP) is the subject of
several studies in regenerative medicine. The aim of this study was to compare four different methods of
preparation of autologous platelet concentrates in sheep. Various specimens were collected from six adult
healthy male sheep of the local breed. Blood smears were also carried out on all the samples, in order to see the
morphology of the platelets. The data obtained in the manual platelet count were submitted to statistical
analysis (repeated measures ANOVA, Tukey HSD test, P<0.05). The result was that the total mean number of
platelets in whole blood was 521.56 ± 97.01x10 /µl. The PRP samples in Groups I and III showed significantly 3
higher mean platelet counts than Group II and IV (1438.25 ± 221.72×10 /µl and 1430.75 ± 293.63×10 /µl, 3 3
respectively). In conclusion, within the limit of this study, it can be concluded that the best technique of platelet
harvesting in sheep is obtained with the double centrifugation protocol used for animals of group I.
 

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