Publications

2024
Mansouri L.M. KA. Salinity effects on germination of Portulaca oleracea L.: A multipurpose halophyte from arid rangelands. effects on germination of Portulaca oleracea L.: A multipurpose halophyte from arid rangelands. Journal of Applied Research on Medicinal and Aromatic Plants [Internet]. 2024. Publisher's Version
Zeroual S., Benaziza A. AFKA(2024). Evaluation of Morphological Characteristics in some Apple Varieties (Malus pumila Mill.) from Northeastern Algeria. Annals of "Valahia" University of Târgovişte. Agriculture [Internet]. 2024. Publisher's Version
A. K. On the observation of nesting of Delta dimidiatipenne (de saussure, 1852) (Hymenoptera: Vespidae: Eumeninae) from North-West Algeria. Acta Musei Silesiae, Scientiae Naturales [Internet]. 2024. Publisher's Version
2023
A. K. First Record of Colchicum triphyllum Kunze (Colchicaceae) from High Altitude in Northeastern Algeria. Acta Horti Bot. Bucurest [Internet]. 2023. Publisher's Version
Kheloufi A., Mansouri L.M. MF. Effect of Salinity on the Germination of Three Species of the Acacia Genus (A. karroo, A. saligna and A. tortilis). Acta Universitatis Sapientiae Agriculture and Environment [Internet]. 2023. Publisher's Version
Djelilate M., Mansouri L.M. MAKA. Lactic Acid Bacteria (Leuconostoc mesenteroides) as Bioprotective Agent against some Pathogenic Fungi in Common Bean. Acta Universitatis Sapientiae Agriculture and Environment [Internet]. 2023. Publisher's Version
Mansouri L.M., Kheloufi A. BM. Aristolochia baetica L. (Aristolochiaceae): New records of rare medicinal species from northwestern Algeria. Contemporary Agriculture [Internet]. 2023. Publisher's Version
Mansouri L.M. KA. Improvement of germination in Retama sphaerocarpa and Ziziphus lotus for the rehabilitation of degraded rangelands in Algeria. Annals of "Valahia" University of Târgovişte. Agriculture [Internet]. 2023. Publisher's Version
2020
Kheloufi A. MLM. Effect of seawater irrigation on germination and seedling growth of Carob tree (Ceratonia siliqua L.) from Gouraya National Park (Béjaïa, Algeria). Reforesta [Internet]. 2020. Publisher's Version
Kheloufi A, Mansouri LM, Khettache H. SEED GERMINATION AND SEEDLING ESTABLISHMENT OF CHERIMOYA (ANNONA CHERIMOLA MILL.) AT DIFFERENT TEMPERATURES. Cercetări Agronomice în Moldova [Internet]. 2020;53 (2) :185-194. Publisher's VersionAbstract
Cherimoya (Annona cherimola Mill.) has an exceptional flavor and aroma, which makes it a fruit with great potential. However, little is known about its propagation by seeds. According to the scientific literature, the germination of cherimoya seeds is affected much more by external conditions than by internal conditions. Germination of cherimoya variety ‘Concha Lisa’ were tested for germination at constant temperatures of 25, 30, 40°C, and at room temperature, varying from 20-25°C, coupled with total darkness. Seeds were sown in Petri dishes (0.8% agar water), for 25 days of incubation. The kinetics of germination was determined according to five closely related parameters, viz. final germination percentage (FGP), mean germination time (MGT), coefficient of velocity of germination (CVG), time to 50% germination (T50) and seedling length (SL). The temperature of 30°C was found optimally suitable with 70.8% FGP, 17.5 days MGT and 3.91 cm SL, while the room temperature of 20-25°C slightly improved germination with only 25% FGP. Furthermore, significant decrease in FGP and SL was observed at 25°C and 40°C of temperature in comparison to 30°C. The analysis also revealed that cherimoya seed germination, day 10-15 after seed sowing is suitable for final counts. An overview on the emergence of cherimoya seedlings, during a 12-week period in pots is presented.
ca2-20-06.pdf
Kheloufi A, Mansouri LM, Laib K. EFFECT OF COLD STRATIFICATION ON SEED GERMINATION OF THE MULTIPURPOSE FRUIT SHRUB, ZIZIPHUS LOTUS (L.) LAM. (RHAMNACEAE). Cercetări Agronomice în Moldova [Internet]. 2020;53 (2) :152-159. Publisher's VersionAbstract
Shrubs and trees of the genus Ziziphus are a good example of naturally occurring multipurpose plant species with great potential in arid regions. This study was conducted to investigate the effect of cold stratification on seed germination and seedling growth of Ziziphus lotus (L.) Lam. Seeds were subjected to 0, 45, 90 and 120 days of cold stratification at 5°C. We also examined fruit, kernel and seed morphology. For each treatment period, four replicates of 50 seeds were incubated in plastic containers between two layers of moist sand at 15% and under greenhouse conditions for 15-day period. At the end of the experiment, the final germination percentage (FGP), shoot length and root length were assessed. The results clearly indicated that increasing duration of cold stratification improved seed germination. The most effective stratification period was 120 days where Z. lotus recorded 83% of FGP and 16.5 cm of total seedling length. Cold stratification treatments significantly increased shoot height, root length, as well as seedling total length. 120 days stratification treatment resulted in the highest shoot and root length (6.80 cm and 9.75 cm, respectively). An overview on the emergence of Z. lotus seedlings during a 15-day period was also illustrated.
ca2-20-03.pdf
Mansouri LM, Kheloufi A, Belatreche R, Heleili N, Boukhatem FZ. EFFECT OF NITROGEN FERTILIZER ON GROWTH, FLOWERING, FRUITING AND NODULATION OF THREE VARIETIES OF COMMON BEAN IN THE ARID REGION OF AÏN NAGA (BISKRA, ALGERIA). Cercetări Agronomice în Moldova [Internet]. 2020;53 (1) :19-35. Publisher's VersionAbstract
Field experiments were conducted to investigate the response of common bean (Phaseolus vulgaris L.) to nitrogen fertilizer. The main factor included the fertilizer (Granular Urea 46% N), was made up of two levels: no fertilization (0 kg/plot: control) and fertilization (0.4 kg/plot), while the secondary factor was the variety (three varieties of common bean: Djedida, Nelson and Jalila). The experimental design was a randomized complete block design with split plot arrangement and replicated four times. The effect of fertilization was evaluated during two development stages (flowering and fruiting) for each variety and this on the total dry biomass, the length of the aerial and root parts, as well as on the number of secondary roots. On the other hand, the combined effect of fertilization and variety was studied on some yield parameters per plant, such as the number of flowers, pods, seeds and the harvest index. In addition, the number of nodules at the end of seed maturation was evaluated. The results indicated that the nitrogen fertilizer application significantly reduced the root length, the number of nodules and secondary roots in most of the common bean varieties. However, fertilizer application significantly increased dry matter in both flowering and fruiting stages, for the three studied varieties. Pod number per plant and seed yield was increased by the application of N fertilizer, depending on varieties and the parameters being measured. Djedida and Jalila varieties gave the best yield and can therefore be recommended to farmers. According to our results, the percentage of improvement by N fertilization on one parameter or another does not exceed an average of 20%, compared to the control. Indeed, the effect of fertilization is positive and seems to increase the harvest index by 18% in Djedida and by 20% in Jalila, compared to non-fertilized plants. Unfertilized plants of the variety Nelson showed the highest ability to nodulate.
ca1-20-02.pdf
Kheloufi A, Mansouri LM, Zerrouni R, Abdelhamid O. Effect of temperature and salinity on germination and seedling establishment of Ailanthus altissima (Mill.) Swingle (Simaroubaceae). Reforesta [Internet]. 2020;9 :44-53. Publisher's VersionAbstract
The tree of heaven Ailanthus altissima (Mill.) Swingle is a multipurpose tree in forestry. However, it is considered an invasive and dangerous plant for native species, and in particular for national parks, where many studies have recorded their involvement in the disturbance of the already developed floral diversity. Assessing the impact of certain abiotic conditions on this species may identify the expected areas to be colonized by its seed propagation. Germination of tree of heaven were tested for germination at constant temperatures of 25, 30, 40°C, and at room temperature varying from 25-30°C coupled with total darkness. Seeds were sown in Petri dishes (0.8% agar water) for 6 days of incubation. The kinetic of germination was determined according to five closely related parameters viz. final germination percentage (FGP), mean germination time (MGT), coefficient of velocity of germination (CVG), time to 50% germination (T50) and seedling length (SL). For the saline condition, the seeds underwent various NaCl concentrations from 0, 50, 100 or 200 mM. For each treatment, there were four replicates with 50 seeds incubated in a plastic container between two layers of moist sand at 15% of the appropriate treatment and then placed in a culture chamber at 27°C (± 2°C) for 30-day period. The effect of temperature was not significant on the MGT, CVG and T50. However, it was significant (p< 0.0001) on FGP and SL. The maximum germination of 94% was obtained at a temperature of 30°C and the lowest FGP of 40% was obtained at 25°C. For the salinity effect, the FGP of 75% in the control was much higher compared to the seeds treated at 50 mM NaCl with only 17.2% of FGP.  Germination was completely inhibited from 100 mM NaCl. A. altissima can be classified as sensitive to salt stress during seed germination and seedling emergence. The salinity effect then joined the temperature to monitor nature's A. altissima seed propagation
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Kheloufi A, Boukhecha M, Ouachi A. Effect of pre-soaking substrate and light availability on seed germination and seedling establishment of Dracaena draco (L.) L., a threatened species. Reforesta [Internet]. 2020;9 :20-29. Publisher's VersionAbstract
The Dragon tree, Dracaena draco (L.) L., is an extremely threatened species. Its natural regeneration is disturbed and population appears to decrease rapidly. To design appropriate germination and seedling establishment protocols for a species conservation and restoration program, we studied the effects of pre-soaking, substrate and light availability treatment on seed germination for 15-day period. The condition for light availability corresponded to the sowing depth (at surface or at 2 cm depth). Germination occurred better in the dark when seeds were sown at 2 cm depth.  Our results showed that D. draco seeds sown in the sand under dark condition and after 24 hours pre-soaking in warm water germinated better and recorded 82.5% of final germination percentage (FGP). However, seedlings grew better in potting soil and recorded 54 mm of length. The maximum FGP of 51.3% was recorded under light condition. These results indicate that sowing depth and light availability could be a limiting factor for D. draco seed germination in natural condition. Furthermore, the establishment of D. draco seedlings (shoot and root length) during an 8-week period in pots was also assessed and illustrated. More research on seed ecophysiology is required to understand the mechanisms controlling seed germination of D. draco in arid and semi-arid conditions viz. salinity and drought.
124-article_text-833-1-10-20200630.pdf
Kheloufi A, Mansouri LM, Telali I, Slimani S. Effect of temperature on seed germination of two Callistemon species (Myrtaceae). Reforesta [Internet]. 2020;9 :1-8. Publisher's VersionAbstract

The genus Callistemon belongs to the family Myrtaceae and comprises 34 species. They are woody aromatic trees or shrubs that are widely distributed in the wet tropics. According to the scientific literature, research on seed germination of this genus was rarely carried out. The germination requirements of Callistemon citrinus and Callistemon linearis were studied under controlled conditions in the laboratory. Seeds of both species were tested for germination at constant temperatures of 25°C, 30°C, 40°C, and at room temperature (23 ± 2°C) coupled with total darkness. Seeds were sown in Petri dishes (0.8% agar water) for 25 days of incubation. The kinetics of germination was determined according to four closely related parameters viz. final germination percentage (FGP), mean germination time (MGT), coefficient of velocity of germination (CVG) and time to 50% germination (T50). The room temperature of 23 ± 2 °C was found optimally suitable for both species, with 80% FGP and 11.1 days MGT for C. citrinus, and only 21.7% FGP and 12.4 days MGT were recorded for C. linearis. Furthermore, significant decrease in FGP was observed in both species at 25°C, 30°C and 40°C of temperature. The analysis also revealed that day 12-15 after seed sowing is suitable for final counts. Due of its very low FGP, seeds of C. linearis need an additional study to determine their viability.

121-article_text-831-1-10-20200630.pdf
Zouagri I, Kheloufi A, Bekhouche F. SEED GERMINATION AND MORPHOPHYSIOLOGICAL CHARACTERIZATION OF THREE TOMATO (LYCOPERSICON ESCULENTUM) VARIETIES IRRIGATED WITH TREATED WASTEWATER . Cercetări Agronomice în Moldova [Internet]. 2020;52 (4) :365-378. Publisher's VersionAbstract
The ability to reuse treated wastewater (TWW) would be of significant benefit to agriculture whilst at the same time providing a valuable water resource. This study concerned the effects of three various levels of treated wastewater (0%, 50% and 100% TWW) on seed germination and plant development of three different varieties of tomato (Lycopersicon esculentum) (viz. Toufan, Heinz and Bouzina). Irrigation with TWW persisted 15 days for the germination stage and 120 days for the growth and development stage. A control plot irrigated with a pure water (groundwater) was also set up in order to compare the seeds and the plants response to different concentrations of irrigation water. The final germination was expressed as a percentage of the total number of seeds in each treatment. This study has demonstrated that treated wastewater improves the germination in Heinz variety and had no effect on both Toufan and Bouzina varieties whether it is pure or diluted by half. For the growth stage, the statistical analysis showed that the Toufan variety has tolerated successfully the TWW irrigation with its high and moderate concentrations, and this by analysing all the morphophysiological parameters studied in this work (leaves numbers, stem and root length and dry biomass, relative water content and rate water loss). 
ca4-19-05.pdf

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